![]() ![]() Extinction Coefficient Calculation - The extinction coefficient is calculated with the following method: ε260 = x 0.9, to adjust for hyperchromicity. The OligoSpec calculator outputs the physical properties for a particular oligo design. Reviewed and updated April 27, 2021.Counter ion MW calc: Important notes If you have any comments, queries, or suggestions, get in touch using the comment box below. Want all your helpful DNA equations in one place in the lab? Download our free lab math cheat sheet for a handy formula and lots of helpful calculations. Once you know the length of your DNA fragment and the concentration in nanograms, you can easily apply the formula to get the number of copies in any type of DNA sample. Note: It’s important to remember that this formula is based on the assumption that you are working with a single DNA species, so when applying to PCR products or plasmids, make sure you only have 1 band or that you have a clean plasmid miniprep. Using Avogadro’s number, which is 6.022 x 10 23 molecules/mole, the number of molecules of the template per gram can be calculated using the following formula: Now we know how to calculate the molecular weight of a DNA template. Question: What is the molecular weight of a 5.2-kb plasmid? Remember 5.2 kb = 5200 bpĬalculation: 5200 bp x 650 daltons = 3,380,000 daltons or g/mol If you need some help with converting your calculations between different units, check out our guide for solving your lab math problems. ![]() ![]() To get the length of your sequence in bp, count the nucleotides in the DNA sequence – either by hand or using character count in a word processing program. the length/size of the genome), this calculation will work for the genomic DNA of any species. The molecular weight or molar mass of any double-stranded DNA fragment can therefore be calculated by multiplying its length (in bp) by 650 and the answer will be expressed as daltons or g/mol.Īs long as you know the sequence length (i.e.This is the same as saying that one mole of a bp weighs 650 g. ![]() This can also be written as 650 g/mol (= molar mass).
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